Lipid-based Nanoparticles
Lipid-Based Nanoparticles (LNPs): Advancing Drug Delivery Science
Manufacturing Lipid Nanoparticles for Therapeutic Applications
Lipid-Based Nanoparticles (Lb-NPs) play a pivotal role in modern pharmaceutical formulations, particularly in the development of vaccines, gene therapies, oncology treatments, and advanced drug delivery systems. The high efficacy of LNP-based mRNA vaccines, such as those used for COVID-19, has further highlighted the importance of precise manufacturing and process control to ensure stability, efficacy, and scalability.
The Importance of Particle Size in LNP Manufacturing
Particle size is a critical quality attribute (CQA) in LNP production, directly influencing:
- Drug encapsulation efficiency and release profile
- Cellular uptake and biodistribution
- Immune response modulation (for vaccines and adjuvants)
- Long-term stability and storage conditions
Ensuring a narrow and well-defined particle size distribution is essential for regulatory compliance and therapeutic effectiveness. However, conventional size characterization methods are offline and time-consuming, limiting their application in real-time process monitoring.
“Continuous processing and enhanced control of lipid-based nanoparticles: LNP’s and liposomes”
Applications of LNPs in the Pharmaceutical Industry
LNP technology is widely applied across various therapeutic domains:
- mRNA and DNA vaccines: Efficient delivery of genetic material to target cells
- Oncology: Lipid-based carriers for chemotherapeutic drugs to enhance efficacy and reduce toxicity
- Gene Therapy: Delivery of siRNA and other nucleic acids for targeted disease treatment
- Adjuvants: Enhancing immune response in vaccine formulations
- Solubilizers: Improving bioavailability of poorly water-soluble drugs
The NanoFlowSizer FIDES: Transforming LNP Manufacturing
To address the challenges in nanoparticle process control, InProcess-LSP introduces the NanoFlowSizer FIDES, a state-of-the-art Spatially Resolved Dynamic Light Scattering (SR-DLS) technology. This PAT tool is uniquely suited for real-time, inline particle size analysis in LNP and liposome manufacturing, offering:
- Non-invasive, continuous size measurement without dilution
- Real-time process insights for batch and continuous production
- Smooth integration with high-throughput manufacturing setups
- Improved regulatory compliance through enhanced process control
By enabling immediate process adjustments, the NanoFlowSizer FIDES ensures that LNP formulations remain within critical quality parameters, reducing batch failures and increasing production efficiency.
With the growing demand for precision nanomedicine, manufacturers require robust, scalable, and real-time process monitoring solutions to meet industry standards.
“Lipid-Based Nanoparticles: Manufacturing and inline size Characterization”
“Integrating the NanoFlowSizer FIDES has significantly reduced batch failures in our vaccine production through precise inline monitoring”
Enabling the Future of LNP Production with Continuous Processing
As the pharmaceutical industry shifts towards continuous manufacturing, real-time process control becomes even more critical. Through its collaboration with DIANT Pharma, InProcess-LSP supports end-to-end continuous LNP manufacturing solutions, integrating advanced PAT tools like the NanoFlowSizer to:
- Optimize nanoparticle formation and reduce batch variability
- Ensure consistent product quality through automated feedback control
- Increase process efficiency while minimizing material waste
Enhance Your LNP Manufacturing Process with InProcess-LSP
For pharmaceutical companies seeking greater precision, efficiency, and scalability in lipid nanoparticle production, InProcess-LSP provides cutting-edge process analytical solutions tailored to meet the challenges of modern drug formulation.
The NEXT-level particle analysis technology in liposome research and manufacturing
Rut Besseling, Science Director at InProcess-LSP
Monitoring lipid-based nanoparticles
Real-time measuremenst in highly turbid sample:
- Lipid Nanoparticles, Liposomes, vaccines, mRNA
- Nanoemulsions
- Silica, iron, gold particles
- Protein molecules
- Polymers, inks, coatings
