How do results from different dynamic light scattering instruments compare?
Dynamic Light Scattering is commonly used to rapidly measure the hydrodynamic size and size dispersity of nanoparticles in suspension. Several commercial DLS instruments exist, using a range of illumination wavelengths and detection angles. These varying instrument specifications have an effect on the measured nanoparticle size and polydispersity index, even for relatively monodisperse nanosuspensions. Here, we explain how results from DLS instruments at different wavelengths and scattering angles are related using practical examples, computer simulations and established light scattering theory, in the context of recently introduced novel Spatially Resolved DLS technology using Near InfraRed light.